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3′ end of each of AS primers to increase allelic specificity (See Table 1). The SNP R (G/A) is illustrated as an example, and the other sorts of SNPs can be applied in the same approach. Four primers are required to amplify a larger fragment from template DNA containing the SNP and two smaller fragments representing every of the 2 AS products. Primers are designed in such a approach that the allelic amplicons differ in size and may be resolved by agarose gel electrophoresis. BatchPrimer3 v1.0 implemented a batch module to easily design two units of tetra-primers for a SNP. The SNP R (G/A) is presented for instance, and the other varieties of SNPs may be applied in the same approach. Four primers, one pair of inner allele-specific (AS) primers and one pair of outer normal primers, are required in a single PCR reaction. Two AS merchandise, one for the G allele and the other for the A allele are amplified using two pairs of primers.
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